moclo plant tool kit Search Results


93
Addgene inc cidar moclo parts kit
Cidar Moclo Parts Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo tool kit
Moclo Tool Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo plant parts kit
Moclo Plant Parts Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo ytk plasmid kit
Moclo Ytk Plasmid Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc cidar moclo extension
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Cidar Moclo Extension, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc yeast modular cloning toolkit moclo ytk
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Yeast Modular Cloning Toolkit Moclo Ytk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc cidar moclo kit
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Cidar Moclo Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo pichia toolkit
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Moclo Pichia Toolkit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo-yeast toolkit
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Moclo Yeast Toolkit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc moclo crispr/cas toolkit for plants
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Moclo Crispr/Cas Toolkit For Plants, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc ecoflex moclo system
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Ecoflex Moclo System, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
New England Biolabs moclo kit
Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a <t>CIDAR</t> cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.
Moclo Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a CIDAR cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.

Journal: ACS synthetic biology

Article Title: Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria.

doi: 10.1021/acssynbio.3c00104

Figure Lengend Snippet: Figure 2. OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a CIDAR cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.

Article Snippet: CIDAR MoClo Extension, Volume I was a gift from Richard Murray (Addgene kit #1000000161). pSEVA331Bb was a gift from Tom Ellis (Addgene plasmid #78269; http://n2t.net/addgene:78269; RRID:Addgene_78269).

Techniques: Cloning, Expressing, Plasmid Preparation, Construct